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Fluorescence emission and fluorescence polarization assays Fluorescence emission and fluorescence polarization spectra for assessment of rucarparib binding: To assess rucaparib binding, rucaparib dissolved in DMSO was mixed with proteins in PBS buffer (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4) to a final DMSO concentration below 2%, and incubated for 5 min prior to measurement
Wang X, Song X, Zhuo W, Fu Y, Shi H, Liang Y, Tong M, Chang G, Luo Y
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